Table 2.
EU specifications for neohesperidine dihydrochalcone (E 959) according to Commission Regulation (EU) No 231/2012
| Commission Regulation (EU) No 231/2012 | |
|---|---|
| Synonyms | Neohesperidin dihydrochalcone; NHDC; Hesperetin dihydrochalcone‐4′‐β‐neohesperidoside; Neohesperidin DC |
| Definition | It is obtained by catalytic hydrogenation of neohesperidin. |
| Einecs | 243‐978‐6 |
| Chemical name | 2‐O‐α‐l‐rhamnopyranosyl‐4′‐β‐d‐glucopyranosyl hesperetin dihydrochalcone |
| Chemical formula | C28H36O15 |
| Molecular Weight | 612.6 |
| Assay | Content not less than 96% on the dried basis |
| Description | Off‐white, odourless, crystalline powder. Approximately between 1,000 and 1,800 times as sweet as sucrose |
| Identification | |
| Solubility | Freely soluble in hot water, very slightly soluble in cold water, practically insoluble in ether and benzene |
| Ultraviolet absorption maximum | 282–283 nm for a solution of 2 mg in 100 mL methanol |
| Neu's test | Dissolve about 10 mg of neohesperidine DC in 1 mL methanol, add 1 mL of a 1% 2‐aminoethyl diphenyl borate methanolic solution. A bright yellow colour is produced |
| Purity | |
| Loss on drying | Not more than 11% (105°C, 3 h) |
| Sulphated ash | Not more than 0.2% (expressed on dry weight basis) |
| Arsenic | Not more than 3 mg/kg (expressed on dry weight basis) |
| Lead | Not more than 2 mg/kg (expressed on dry weight basis) |