Figure 4.

AMPK knockdown alleviated HOP-induced mitochondrial fission and mitophagy. (A) Protein expression of AMPK, p-MFF, and LC3 in transfected siAMPK and siNC cells with or without exposure to HOP. (B) Quantitative analysis of LC3 protein expression in HCECs. (C) Immunofluorescence analysis of DRP1 and Tom20 co-localization after AMPK knockdown. Scale bar: 20 µm. (D) Mitochondrial morphology of HCECs under a confocal microscope after AMPK knockdown. Scale bar: 10 µm. (E) Immunofluorescence images showing the co-localization of LC3B and MitoTracker Deep Red. Scale bar: 20 µm. Results are presented as mean ± SD from three independent experiments. **P < 0.01, ****P < 0.0001.