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. 2022 Nov 14;63(12):18. doi: 10.1167/iovs.63.12.18

Figure 6.

Figure 6.

Knockdown of MFF alleviated HOP-induced mitochondrial fragmentation and mitophagy. Immortalized HCECs were transfected with MFF-specific shRNA or blank lentiviral vectors. The stably transfected HCECs were exposed to normal or hyperosmotic medium for 36 hours. (A) Western blot results of p-MFF, MFF, and LC3 expression in shMFF knockdown HCECs with or without HOP treatment. (B, C) Quantitative analysis of p-MFF and LC3 expression in HCECs following shMFF knockdown. (D) Immunofluorescence co-localization analysis of DRP1 and Tom20 in HCECs following shMFF knockdown. Scale bar: 10 µm. (E) Quantitative analysis of DRP1 co-localization with Tom20 in HCECs after shMFF knockdown. (F) Immunofluorescence images of LC3 localized on mitochondria in HCECs after shMFF knockdown. Scale bar: 10 µm. Results are presented as the mean ± SD of three independent experiments. **P < 0.01,***P < 0.001.