Figure 7.
Knockdown of MFF alleviated increased ROS and inflammatory factors under hyperosmolar conditions. HCECs transfected with blank and shRNA of MFF lentiviral vectors were exposed to normal or hyperosmotic medium for 24 hours. (A) The results of intracellular ROS in HCECs by H2DCFDA assay. Scale bar: 50 µm. (B) Statistical results of the mean fluorescence intensity of intracellular ROS in HCECs. (C) Changes to cell viability by CCK-8 assay following MFF knockdown. (D) Results of RT-PCR for gene expression of intracellular inflammatory factors NLRP3 and TNF-α. Three independent experiments were conducted, and results are presented as mean + SD. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001.