Skip to main content
. 2022 Nov 3;13:940475. doi: 10.3389/fphar.2022.940475

FIGURE 4.

FIGURE 4

Eupatilin attenuates TNF-α-induced ECM degradation and improves the senescence of NP cells via inhibition of the MAPK/NF-κB signaling pathway. (A) Western blot was used to detect the expression of MMP9, MMP13, SOX9, and COL2A1 proteins in the cytoplasm after treatment with TNF-α (20 ng/ml) with or without eupatilin (6.25 μM, 12.5 μM) for 24 h. (B) According to the results, proteins in (A) were statistically analyzed (n = 3). (C) Western blot was performed on the effects of eupatilin on the phosphorylation of IκBα and p65, and total IκBα induced by TNF-α. NP cells were pretreated with eupatilin (6.25 μM, 12.5 μM) for 2 h before the stimulation with TNF-α (20 ng/ml) for 20 min. (D) Statistical analysis (n = 3) according to the results in (C). (E) Western blot was performed on the effects of eupatilin on the phosphorylation of p38, RRK, and JNK induced by TNF-α. NP cells were pretreated with eupatilin (6.25 μM, 12.5 μM) for 2 h before the stimulation with TNF-α (20 ng/ml) for 20 min. (F) Statistical analysis (n = 3) according to the results in (E). Data are presented as the mean ± SD. n = 3, compared with the TNF-α-alone group. *p < 0.05, **p < 0.01, ***p < 0.001, and ****p < 0.0001; ns: the difference was not statistically significant.