TABLE 1.
High‐pressure size exclusion chromatography columns
| SD120 5/150 no. 6 | SD300 5/300 no. 1 | SD300 5/300 no. 3 | SD300 5/300 no. 4 | SD300 5/300 no. 7 | |
|---|---|---|---|---|---|
| Packing type | Silica diol | Silica diol | Silica diol | Silica diol | Silica diol |
| Pore size (Å) | 120 | 300 | 300 | 300 | 300 |
| Particle size (μm) | 5 | 5 | 5 | 5 | 5 |
| pH range | 5.0–7.5 | 5.0–7.5 | 5.0–7.5 | 5.0–7.5 | 5.0–7.5 |
| Mol. wt. range (kDa) | 1–100 | 20–1,000 | 20–1,000 | 20–1,000 | 20–1,000 |
| Column dimensions (mm) | 4.6 × 150 | 4.6 × 300 | 4.6 × 300 | 4.6 × 300 | 4.6 × 300 |
| Frit porosity (μm) | 0.5 (PEEK) | 0.5 (PEEK) | 0.5 (PEEK) | 0.5 (PEEK) | 2.0 (SS) |
| Column volume (cm3) | 2.49 | 4.99 | 4.99 | 4.99 | 4.99 |
| Void volume (cm3) | 0.97 | 2.94 | 2.21 | 3.63 | 1.54 |
| Acetone pulse (cm3) | 2.14 | 4.36 | 4.48 | 4.39 | 4.27 |
Abbreviations: PEEK, polyether ether ketone; SS, stainless steel.