. Author manuscript; available in PMC: 2022 Nov 17.

Published in final edited form as: SLAS Discov. 2022 Mar 21;27(4):242–248. doi: 10.1016/j.slasd.2022.03.005

Table 1.

NR2F6 assay 1536 High-Throughput Screening Campaign

	Screen Stage	Target	No. Tested	Hit Cutoff^a	No. Selected	Z’	Signal: Background
Pilot	LOPAC Pilot 3X	NR 2F 6	1280	77.75% ^b	8 (0.21%)	0.71 ± 0.06	3.68 ± 1.27
1	Primary	NR 2F 6	666,120	43.87% ^b	5008 (0.75%)	0.64 ± 0.07	148.04 ± 12.36
2a	Confirmation 3X	NR 2F 6	4994	46.87%^*	1925 (38.55%)	0.63 ± 0.04	182.81 ± 14.48
2b	Counter screen 3X	Empty vector	4994	21.09%^*	2334 (46.74%)	0.77 ± 0.03	166.24 ± 14.48
3a	Titration 3X	NR 2F 6	498	IC50 < 5µM	270	0.61 ± 0.06	197.97 ± 13.67
3b	Titration 3X	Empty vector	498	IC50 > 10µM	192	0.76 ± 0.04	215.84 ± 7.54

The concentrations used were 9.9∞M for steps 1, 2a, and 2b; 25.2∞M titration starts for titration assays

Standard-based cutoffs

DMSO sample field cutoff