. Author manuscript; available in PMC: 2023 Oct 18.

Published in final edited form as: Biochemistry. 2022 Sep 22;61(20):2221–2228. doi: 10.1021/acs.biochem.2c00342

Table 1.

MdG⁸⁹ Hydrolysis in Free DNA and NCPs

substrate	k_Hyd (10⁻⁷ s⁻¹)^a	t_1/2 (h)	rel. t_1/2^b
free DNA^c	10.1 ± 0.5	191 ± 8
WT NCP^c	3.5 ± 0.2	553 ± 27	2.9
H4-K16,20A	4.6 ± 0.2	417 ± 18	2.2
H4-K16,20A, R17,19A	5.8 ± 0.1	330 ± 7	1.7
H4 Del 1–20	5.9 ± 0.5	330 ± 30	1.7

Values are the ave. ± std. dev. of three independent experiments.

Rel. t_1/2 = t_1/2 (NCP)/t_1/2 (Free DNA).

Ref 18.