FIGURE 7.

PAK5‐regulated phosphorylation facilitates the nuclear translocation of SRSF11 and inhibits the inclusion of HSPA12A exon 2. (A) Representative immunofluorescence images by confocal microscope indicate SRSF11 protein localization in LoVo cells. (B) WB analysis of SRSF11 protein levels in cytoplasm and nucleus, respectively. β‐actin and Lamin‐B1 were used as cytoplasmic and nuclear internal controls, respectively. (C) RT‐PCR results and quantification of HSPA12A RNA products measured as PSI after co‐transfection with indicated plasmid and siRNA in LoVo and SW480 cell lines (N = 3, SNK test; *p < .05, **p < .01, ***p < .001)