Figure EV1. Dynamics of H3K27ac in mouse oocytes and early embryos.

1. Spearman correlation of H3K27ac CUT&RUN replicates and correlation with available public data. The public H3K27ac data were generated using the μChIP–seq method (Dahl et al, 2016). GV—germinal vesicle oocyte; FGO—fully grown oocyte; 2C—2‐cell embryo.
2. Scale factors for H3K27ac FPKM at different stages. The scale factors at top 3,000 promoters were used.
3. H3K27ac domain bases distribution at promoter, exon, intron, and intergenic regions for each stage.
4. Dynamic changes of H3K27ac from early 2‐cell to morula stage and ESC. C: domain center.
5. Correlations between gene density and H3K27ac signals for each stage.
6. H3K27ac signal enrichment around TSS of genes with CGI or non‐CGI promoters. CGI: CpG island.
7. Enrichment of H3K27ac at ERVL retrotransposons at different stages.
8. Genome browser view showing different dynamics of H3K27ac and H3K9ac from zygotes to late 2‐cell embryos.