Figure EV3. Transcriptome comparison of CBP/p300 inhibition versus control.

1. Expression level dynamics of Ep300 (p300) and Crebbp (CBP) in MII oocyte and preimplantation embryos.
2. Determination of optimal A‐485 concentration to inhibit CBP/p300 by H3K27ac immunostaining. The embryos (3 biological replicates in each condition) were treated with A‐485 starting at 4 hpi, and images were taken at 7 hpi. Scale bar: 20 μm.
3. Immunostaining quantification of H3K27ac relative intensities in Fig 4B (6–12 biological replicates in each condition).
4. Pearson correlation of RNA‐seq replicates at zygote, early 2‐cell, and late 2‐cell stage for control (DMSO‐treated) and CBP/p300 inhibition (A‐485‐treated).
5. Gene Ontology (GO) enrichment for the down‐regulated genes at early 2‐cell stage after A‐485 treatment.
6. Scatter plot showing the expression level changes of repeat elements after CBP/p300 inhibition.

Data information: For boxplots in (B) and (C), the central band represents the median. The lower and upper edges of the box represent the first and third quartiles, respectively. The whiskers of the boxplot extend to 1.5 times interquartile range (IQR).