Table 3. Apparent Steady-State Kinetic Constants for the Oxidation of L-Lactate by Various LDHsa.
| Km [mM] | kcat [s–1] | kcat/Km [mM–1 s–1] | |
|---|---|---|---|
| LjLDH | 1.35 ± 0.18 | 47.2 ± 2.0 | 35.0 |
| LhLDH | 0.52 ± 0.09 | 102 ± 8 | 196 |
| SsLDH | 5.67 ± 1.24 | 107 ± 10 | 18.9 |
| GbLDH | 16.9 ± 2.5 | 207 ± 29 | 12.3 |
| EaLDH | 21.6 ± 2.2 | 94.0 ± 7.8 | 4.35 |
| PaLCTO | 235 ± 45 | 61.8 ± 8.5 | 0.263 |
a
Reactions were measured in 11 mM PBS pH 7.4 at 30 °C and DCIP as the electron acceptor used at a constant concentration of 120 μM. L-Lactate concentrations were varied from 0.125 to 64 mM or from 4 to 500 mM for PaLCTO.