TABLE 2.
Comparison between fresh and frozena PBMC for their proliferative responses to B. pertussis antigens in 48-month-old children
| Antigen | Lymphoproliferative responseb
|
|||
|---|---|---|---|---|
| CMI+
|
CMI−
|
|||
| Fresh | Frozen | Fresh | Frozen | |
| None | 0.5 ± 0.1 | 0.35 ± 0.07 | 0.4 ± 0.0 | 0.2 ± 0.05 |
| PT | 21.4 ± 1.3 | 32.7 ± 5.7 | 2.8 ± 1.1 | 2.1 ± 1.5 |
| PRN | 16.9 ± 2.4 | 35.2 ± 9.9 | 1.1 ± 0.4 | 0.7 ± 0.1 |
| FHA | 12.1 ± 1.8 | 18.3 ± 0.8 | 0.8 ± 0.1 | 0.4 ± 0 |
| BPc | 10.0 ± 2.3 | 20.1 ± 6.0 | 1.3 ± 0.0 | 1.7 ± 0.3 |
a
Fresh and frozen PBMC from four children (two CMI+ and two CMI−) were assayed for proliferation as described in Materials and Methods and in the legend to Table 1.
b
Expressed as mean (triplicate samples from both children) ± SE of thymidine incorporation values (103 cpm).
c
B. pertussis cells (strain 18323; ATCC 9797) were heat inactivated (96°C; 1 h) and used at a concentration of 106 CFU/ml.