KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit polyclonal anti-Nesprin-2 | Gift of Dr. Gundersen [23] | N.A. |
| Rabbit polyclonal anti-HA | Sigma-Aldrich | Cat#6908 |
| Mouse monoclonal anti-DIC 74.1 | Univ. of Virginia | N.A. |
| Rabbit monoclonal anti-BicD1 | Abcam | Cat#170878 |
| Rabbit polyclonal anti-BicD2 | Abcam | Cat#117818 |
| Rabbit polyclonal anti-BicD2 CT | GeneTex | Cat#120683 |
| Rabbit polyclonal anti-Nde1/Ndel1 | [59] | N.A. |
| Mouse monoclonal anti-Cyclin B1 | Biosciences | Cat#554177 |
| Chicken polyclonal anti-GFP | Millipore | Cat#16901 |
| Rabbit polyclonal anti-GFP | Invitrogen | Cat#11122 |
| Mouse monoclonal anti-GST | Santa Cruz | Cat#53909 |
| Chemicals, Peptides, and Recombinant Proteins | ||
| Aqua-Poly mounting media | Polysciences | Cat#18606 |
| Low melting agarose | IBI Scientific | Cat#70057 |
| Penicillin/streptomycin/glutamine | Life Technologies | Cat#10378-016 |
| Agarose | Sigma | Cat#9539 |
| Dye | Sigma | Cat#7252 |
| Normal Donkey Serum | Sigma | Cat#9663 |
| Hanks balanced salt solution | Life Technologies | Cat#24020-117 |
| Basal MEM | Life Technologies | Cat#21010-046 |
| Horse serum | Life Technologies | Cat#26050-088 |
| Glucose | Sigma | Cat#5767 |
| Critical Commercial Assays | ||
| KOD Hot Start DNA Polymerase | Millipore | Cat#71086 |
| Effectene | Qiagen | Cat#301425 |
| Glutathione agarose beads | Affymetrix | Cat#78820 |
| HRV 3C Protease | PierceTM | Cat#88946 |
| Glutathione magnetic beads | Thermo Scientific | Cat#78601 |
| Experimental Models: Cell Lines | ||
| NIH 3T3 | Gift of Dr. Gundersen, Columbia Univ. | ATCC CRL-1658 |
| HeLa | Gift of Dr. Allan, Univ of Manchester | HELAM |
| Experimental Models: Organisms/Strains | ||
| Sprague Dawley Pregnant Rats | Taconic | N.A. |
| Recombinant DNA | ||
| pCAGIG vector | Addgene [57] | Cat#11159 |
| pCAGIG-BicD2CT | This paper | N.A. |
| pCAGIG Mini N2G SR 52–56 | This paper | N.A. |
| pCAGIG N2G SR 52–56 | This paper | N.A. |
| pCAGIG N2G SR 52–56 LEAA | This paper | N.A. |
| pCAGIG Mini N2G | This paper | N.A. |
| pCAGIG Kif5B | This Paper | Synthesized by Synbio Technologies |
| pCAGIG KLC-TPR | This Paper | Synthesized by Synbio Technologies |
| pCALNL-GFP | Addgene [58] | Cat#13770 |
| pCALNL-BicD2CT | This paper | N.A. |
| pNeuroD1-Cre | Gift of Dr. Cardoso, Institut de Neurobiologie de la Méditerranée | N.A. |
| pRNAT-U6.1/Neo BicD2 | [14] | N.A |
| pGFP-V-RS RanBP2 | [13] | N.A. |
| pRETRO LIC1 | [15] | N.A. |
| mRFP-KASH | [23] | N.A. |
| GST-BicD2-CT | Gift from Dr. Anna Akhmanova, Utrecht University [32] | N.A. |
| His-BicD2-CT | [13] | N.A. |
| GST-N2G | gift from Dr. Gundersen, Columbia University [22] | N.A. |
| pCAG DsRed-PACT | This Paper | N.A. |
| pMSCV-puro GFP-Mini N2G | Gift of Dr. Gundersen, Columbia University [22] | N.A. |
| pMSCV-puro GFP-N2G SR 52–56 | Gift of Dr. Gundersen, Columbia University [22] | N.A. |
| pEGFP-C1 GFP-Mini N2G | Gift of Dr. Gundersen, Columbia University [22] | N.A. |
| pEGFP-C1 GFP-Mini N2G SR 51–26 | Gift of Dr. Gundersen, Columbia University [22] | N.A. |
| pMSCV N2G SR 52–56 | Gift of Dr. Gundersen, Columbia University [22] | N.A. |
| pMSCV N2G SR 52–56 LEAA | Gift of Dr. Gundersen, Columbia University [22] | N.A. |
| Software and Algorithms | ||
| Fiji | Image J | https://fiji.sc |