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Visualizing lipid receptor localization and activation. (A) CB1 and CB2 receptors can be targeted with either a two-step photoaffinity probe or a directly fluorescent probe to reveal its location and occupancy. (B) The genetically encoded eCB2.0 receptor contains a circularly permutated GFP (cpGFP), which will only become fluorescent upon binding 2-AG or AEA to report on endocannabinoid action.
