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. 2022 Oct 13;90(11):e00417-22. doi: 10.1128/iai.00417-22

FIG 3.

FIG 3

TLR2 is dispensable for the control of bacterial burdens and does not contribute to bone damage during S. aureus osteomyelitis. (A–H) Mice were subjected to osteomyelitis via intraosseous injection of 106 CFU of S. aureus. Femurs were extracted on day 14 postinfection. For all graphs, error bars denote the SD. For all graphs, *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001. If not denoted with asterisks, the differences between genotypes were not statistically significant. (A) Femurs, kidneys, and livers were extracted and homogenized for CFU enumeration. Dotted lines indicate the Log10 transformed limits of detection. The Log10 transformed CFU/femur values were compared between genotypes by unpaired t tests. Data were pooled from two independent experiments, n = 10 per genotype. (B–H) Infected and contralateral femurs were isolated. Bone parameters were assessed using microcomputed tomography (μCT). Results are compiled from two independent experiments. For cortical bone, n = 10 for WT and n = 9 for Tlr2−/−. For trabecular bone, n = 9 for WT and n = 10 for Tlr2−/−. (B) Representative 3D images of infected femurs were constructed by μCT. (C) Cortical bone loss was calculated using μCT, and were compared between genotypes using an unpaired t test. (D) Callus formation was measured by μCT, and compared between genotypes using an unpaired t test. (E) Representative images of trabecular bone in infected and contralateral femurs were constructed by uCT. Images represent the median infected femur % bone volume/total volume (%-BV/TV). (F) The %-BV/TV of contralateral femurs was calculated using μCT, and the values were compared using an unpaired t-test. (G) The %-BV/TV values of infected femurs were compared between genotypes using an unpaired t-test. (H) Histomorphometry was performed on TRAP-stained femur sections to measure TRAP+ cell surface on the trabecular bone, relative to total trabecular volume. The %-osteoclast surface/bone surface (Oc.S/BS) was compared between genotypes using a Mann-Whitney U test.