Table 3. Results of Method Evaluationa.
Precision |
Accuracy |
Calibration
Statistics |
||||||
---|---|---|---|---|---|---|---|---|
Analyte | Repeat. | Intra-Assay | Inter-Assay | % Recovery | % Error | LOD | LOQ | Linearity (R2) |
E1 | 12.2 | 11.2 | 9.6 | 102.0 | 8.5 | 0.09±0.03 | 0.27±0.09 | 0.991 ± 0.005 |
24 ± 8 | 72 ± 24 | |||||||
E2 | 11.5 | 10.4 | 8.9 | 103.3 | 7.1 | 0.10±0.04 | 0.30±0.13 | 0.988 ± 0.009 |
27 ± 12 | 82 ± 36 | |||||||
BODIPY-E1 | 1.0 | 1.1 | 1.3 | 96.5 | 4.3 | 0.02±0.01 | 0.07±0.03 | 0.999 ± 0.001 |
14 ± 7 | 42 ± 21 | |||||||
BODIPY-E2 | 1.2 | 1.2 | 1.4 | 95.9 | 4.8 | 0.02±0.01 | 0.07±0.03 | 0.999 ± 0.001 |
13 ± 6 | 40 ± 19 |
Precision values are percent relative standard deviation. The limit of detection (LOD) and limit of quantitation (LOQ) are reported in micromolar (bold, top values) and μg/L (bottom values). The linearity and limit values are the means of the three different batches (n = 3) and include standard deviation (±value).