Table 2.
Summary of miRNA targets that were validated via PARE‐Seq. The described miRNAs were captured in barley microspores (cv. Gobernadora) at different time points of gametic embyrogenesis
| miRNA | Target gene | Orthologous gene | Name | Target function |
|---|---|---|---|---|
| Microspores on day 0 | ||||
| miR168 | HORVU7Hr1G007000 | AT1G48410 | AGO1 | RNA‐mediated gene silencing, Translation regulation |
| miR396 | HORVU6Hr1G084590 | AT1G79690 | NUDT3 | Hydrolase |
| miR1127 | HORVU4Hr1G042610 | AT5G19390 | ROPGAP7 | Signal transduction, Cell wall biogenesis |
| Microspores on day 2 | ||||
| miR1127 | HORVU2Hr1G070360 | AT5G50600 | HSD1 | Lipid biosynthesis, Lipid metabolism, Steroid biosynthesis |
| miR1127 | HORVU3Hr1G078090 | AT3G58830 | T20N10_180 | |
| miR1130 | HORVU4Hr1G064990 | AT2G32840 | Negative regulation of transcription by RNA polymerase II | |
| miR1130 | HORVU6Hr1G057140 | AT2G23360 | FPP7 | Unknown |
| miR1130 | HORVU7Hr1G122690 | AT3G09920 | PIP5K9 | Carbohydrate metabolic process, Cellular amino acid metabolic process |
| Microspores on day 5 | ||||
| miR166 | HORVU1Hr1G041790 | AT5G60690 | REV | Differentiation, Transcription, Transcription regulation |
| miR156 | HORVU3Hr1G094730 | Os01g0922600 | SPL2 | Transcription regulation |
| miR156 | HORVU0Hr1G020810 | Os08g0509600 | SPL14 | Transcription regulation |
| miR396 | HORVU2Hr1G101770 | Os04g0600900 | GRF3 | Transcription regulation |
| miR396 | HORVU0Hr1G016590 | Os02g0701300 | GRF4 | Transcription regulation |
| miR396 | HORVU0Hr1G016610 | Os02g0701300 | GRF4 | Transcription regulation |
| miR396 | HORVU0Hr1G026650 | Os02g0701300 | GRF4 | Transcription regulation |
| miR396 | HORVU6Hr1G068370 | Os02g0701300 | GRF4 | Transcription regulation |
| miR396 | HORVU2Hr1G094470 | Os04g0574500 | GRF12 | Transcription regulation |
| miR1127 | HORVU1Hr1G060530 | AT5G18860 | NSH3 | Response to jasmonic acid |
| miR1130 | HORVU4Hr1G045760 | AT1G18660 | mRNA processing | |
| Common to day 0 and day 2 | ||||
| miR5049 | HORVU7Hr1G085310 | AT4G13360 | Hydrolase, mitochondrial | |
| miR1127 | HORVU2Hr1G086380 | AT5G48930 | HCT | Cell wall biogenesis/degradation, Auxin homeostasis |
| Common to day 0 and day 5 | ||||
| miR1127 | HORVU7Hr1G059130 | AT5G03880 | MER24.18 | Cell redox homeostasis |
| miR1127 | HORVU7Hr1G056530 | AT1G66950 | ABCG39 | Transport |
| Common to day 2 and day 5 | ||||
| miR167 | HORVU2Hr1G121110 | Os04g0671900 | ARF8 | Auxin signaling pathway, Transcription regulation |
| miR396 | HORVU7Hr1G008680 | Os06g0116200 | GRF5 | Transcription regulation |
| miR1127 | HORVU3Hr1G051000 | AT5G20970 | HSP20 | |
| miR1127 | HORVU7Hr1G097520 | AT4G28470 | RPN1B | Protein catabolic process |
| miR1130 | HORVU5Hr1G044640 | AT5G13750 | ZIFL1 | Transport |
| miR5048 | HORVU7Hr1G065130 | AT5G15730 | CRLK2 | Protein phosphorylation |
| miR5048 | HORVU7Hr1G043150 | AT5G15730 | CRLK2 | Protein phosphorylation |
| miR9674 | HORVU0Hr1G031920 | Os04g0350000 | Rf1 | Restorer of fertility |
| miR9674 | HORVU0Hr1G035310 | Os04g0350000 | Rf1 | Restorer of fertility |
| miR9674 | HORVU1Hr1G010890 | Os04g0351333 | Rf1 | Restorer of fertility |
| miR9674 | HORVU1Hr1G010970 | Os10g0497300 | Rf1 | Restorer of fertility |
| miR9674 | HORVU1Hr1G010990 | Os10g0497300 | Rf1 | Restorer of fertility |
| miR9674 | HORVU1Hr1G011020 | Os04g0350000 | Rf1 | Restorer of fertility |
| miR9674 | HORVU1Hr1G011150 | Os04g0350000 | Rf1 | Restorer of fertility |
| miR9674 | HORVU1Hr1G011160 | Os04g0351333 | Rf1 | Restorer of fertility |
| miR9674 | HORVU1Hr1G011250 | Os04g0350000 | Rf1 | Restorer of fertility |
| miR9674 | HORVU1Hr1G011300 | Os04g0351333 | Rf1 | Restorer of fertility |
| Common to day 0, day 2 and day 5 | ||||
| novel.3 | HORVU3Hr1G070880 | AT3G16360 | AHP4 | Cytokinin signaling pathway, Two‐component regulatory system |