Figure 2.
Amplification rate for φ29 DNA polymerase. (A) RCA reaction performed in solution with primer 1:circle 1 duplexes. Total DNA synthesis (filled circles) was measured as acid-precipitable counts. Decoration of RCA product with 32P-labeled probe (open circles). The dashed line indicates the estimated intensity values assuming a maximal rate of extension by the polymerase. Fold amplification was calculated by dividing either the number of tandem repeats (calculated from total DNA synthesis) or the amount of decorator annealed to RCA products by the input template circle. (B) An RCA reaction performed as described in (A) except that the reactions were assembled on glass microarrays printed with primer 1. The RCA product was detected by hybridization with decorator probes and scanning in a laser scanner. Error bars show the averages of three replicate experiments.