Figure 6:
a) Vesicles made of POPC by different preparation methods deform at a different rate indicating different membrane viscosity. The field strength and frequency are 8 kV/m and 0.1 kHz. The solid lines correspond to the theoretical fit with Eq. 2.b) Box-plot figure of shear viscosity values of bilayers obtained with transient electrodeformation for seven different GUV preparation methods. The box-plot represents the standardized distribution of data based on minimum value, first quartile (Q1), mean, third quartile (Q3), and maximum value. The open square represents the mean value. The abbreviations in the figure are as follows, SS: spontaneous swelling, EF: electroformation, PVA: polyvinyl alcohol, PTM (MO): phase-transfer method (mineral oil) and PTM (H): phase-transfer method (hexadecane). ANOVA comparisons test compared to spontaneous swelling which is set as control. ***p≤0.001, **p≤0.01, n.s p≥0.05 c) Viscosity dependence on electric field strength, or equivalently strain rate .