Membrane bending rigidity, capacitance and shear viscosity of POPC bilayers obtained from different preparation methods at 25 °C and determined in this study. Bending rigidity was measured with flickering spectroscopy and membrane capacitance and viscosity was measured with the electrodeformation method. The abbreviations in the table are as follows, SS: spontaneous swelling, EF: electroformation, PVA: polyvinyl alcohol, PTM (MO): phase-transfer method (mineral oil) and PTM (H): phase-transfer method (hexadecane).