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Purification of OaBac5α. The inset photographs are of silver-stained SDS-PAGE gels. Photograph a shows the composition of fractions 21 to 25, obtained after continuous preparative electrophoresis (8). The masses of two standards (3.0 and 6.2 kDa; Std) are shown. The main figure shows a chromatogram of the RP-HPLC purification of electrophoretic fractions 22 and 23. Note the prominent peak (OaBac5α) that emerged at approximately 34% acetonitrile. Photograph b shows this peak.
