Table 1. Concentration levels of components used in a multifactorial array for the identification of discriminatory reaction conditions for bi-directional ASA.
Level | A | B | C |
---|---|---|---|
WT-AS/WT-CP517 (µM) | 0.2 | 0.4 | 0.8 |
MUT-AS/MUT-CP267 (µM) | 0.2 | 0.4 | 0.8 |
MgCl2 (mM) | 0.5 | 1 | 2 |
dNTPs (mM) | 80 | 120 | 200 |