Figure 3.

Voltage-dependent properties of cell-attached macroscopic currents induced by the overexpression of Kv3.4-WT, Kv3.4-PN, and Kv3.4-PM in embryonic DRG neurons. A, Normalized peak conductance - voltage relations of endogenous and overexpressed Kv3.4 channels. The voltage protocol used to generate these relations is shown in Figure 2F. The solid lines are the best-fit first-order Boltzmann functions (see above, Materials and Methods). Best-fit parameters and shown in Table 1. B, Scatter and box plots of the activation V_0.5. C, Scatter and box plots of the activation equivalent gating charge. D, Prepulse inactivation curves of endogenous and overexpressed Kv3.4 channels. The prepulse steady-state inactivation protocol consisted of a varying 20 s conditioning pulse from −120 mV to +80 mV followed by a 300 ms test pulse to +80 mV. The solid lines are the best-fit first-order Boltzmann functions (see above, Materials and Methods). Best-fit parameters are shown in Table 1. E, Scatter and box plots of the inactivation V_0.5. F, Scatter and box plots of the inactivation equivalent gating charge. The upper and lower borders of the boxes indicate the 75th and 25th percentiles, respectively; the line across the box indicates the 50th percentile, and the top and bottom whiskers indicate the maximum and minimum, respectively.