Figure 3.

DNA and NET clearance activities correlate with regulators and disease severity markers in sepsis and COVID-19 pneumonia

(A) Correlation between DNase activity (D₅₀) and DNA (left) or histone H3 (right) in SP samples.

(B) Correlation between DNase I and DNase activity at 10% plasma (left) or NETase activity in SP samples (right).

(C) Correlation between SP plasma actin and DNase activity (D₅₀) (left) or NETase activity (right).

(D) Correlation between SP plasma gelsolin (GSN) and DNase activity (D₅₀) (left) or NETase activity (right).

(E) Correlation between CP plasma actin measured by mass spectrometry and DNase activity (D₅₀) (left) or NETase activity (right).

(F) CP plasma DNase I plotted against DNase activity (D₅₀) (left) or NETase activity (right).

(G) From left to right, HI values, thrombocyte counts, neutrophil to lymphocyte ratio (NLR), and red cell blood distribution width (RDW-CV) plotted against their corresponding NETase activity in plasmas of individuals with COVID-19.

(H) Correlation between raw DNase activity measurements at 10% plasma dilution and CRP (left) or FGG protein (right) in WHO-7 (orange circle) and WHO-3 and -4 (green circle) CP samples.

(I) Correlation between ORM1;ORM2 and DNase activity measurements at 10% plasma dilution (left) or NETase activity (right) in WHO-7 and WHO-3 and -4 CP samples.

(J) Correlation between ORM2 (RPA) and DNase I measured in CP by ELISA. Statistics by Mann-Whitney or Kruskal-Wallis test for single comparisons, one-way ANOVA for multiple comparisons, simple linear or non-linear regression for correlations. See also Figure S3.