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. Author manuscript; available in PMC: 2023 Dec 1.
Published in final edited form as: Osteoarthritis Cartilage. 2022 Sep 6;30(12):1616–1630. doi: 10.1016/j.joca.2022.08.015

Figure 4. Histopathological evaluation of CCR2-AggKO and CCR2+/+ mouse knees, following late CCR2 inactivation.

Figure 4.

(A) H&E staining of the knee medial compartment from CCR2-AggKO and CCR2+/+ mice after DMM/Sham (time indicated); images are representative of N=8 for each time point. (B) ACS score (0–12 scale) of DMM/Sham knees at the time point indicated. Results are expressed as average of 4 quadrants (medial and lateral tibial plateau, medial and lateral femoral condyles); N=8 mice for each experimental point. (C) Safranin-O/Fast green staining of the knee medial compartment after DMM/Sham (time indicated); images are representative of N=8 for each time points. (D) Safranin-O staining Score (0–12 scale) of DMM/Sham knees at the time point indicated. Results are expressed as average of 4 quadrants as described above; N=8 mice for each experimental point. (E) Quantification of the AC area (sq μm) of the medial plateau of DMM/Sham knees by histomorphometric analysis at the time point indicated; N=8 mice for each experimental point. The graphs represents the mean ± standard deviation; indicated p-values were determined by Wilcoxon rank sum tests at each time point, following adjustment for multiple comparisons. Scale bars of the images are 100 μm.