Table 1.
Effect of early CCR2 inactivation (before DMM) on OA assessment and pain in mouse CCR2-AggKO and CCR2+/+ knee joints
| OA parameters | 2 weeks | 4 weeks | 8 weeks | 12 weeks | |||||
| Z (pvalue*) | median | Z (pvalue) | median | Z (pvalue) | median | Z (pvalue) | median | ||
| ACS | DMM CCR2+/+ | 0.63 (0.53) | 0.75 | 1.03 (0.41) | 1.75 | 3.38 (0.002) | 7.63 | 3.52 (0.002) | 6.50 |
| DMM CCR2-AggKO | 0.5 | 0.75 | 3.50 | 4.50 | |||||
| Saf-o | DMM CCR2+/+ | 3.01 (0.004) | 2.00 | 2.42 (0.02) | 2.38 | 3.52 (0.002) | 5.25 | 3.21 (0.002) | 5.88 |
| DMM CCR2-AggKO | 0.89 | 1.00 | 2.75 | 4.00 | |||||
| Cartilage Quantification | DMM CCR2+/+ | −1.52 (0.13) | 20927 | −3.20 (0.004) | 16083 | −2.26 (0.03) | 9712 | −3.20 (0.03) | 8811 |
| DMM CCR2-AggKO | 23874 | 21128 | 15589 | 19264 | |||||
| Osteophyte size | DMM CCR2+/+ | 0.15 (0.88) | 0.63 | 2.02 (0.16) | 1.13 | −1.03 (0.60) | 1.00 | 0.25 (0.88) | 1.13 |
| DMM CCR2-AggKO | 0.63 | 0.88 | 1.25 | 1.00 | |||||
| Osteophyte maturity | DMM CCR2+/+ | 0.77 (0.44) | 0.50 | 3.04 (0.04) | 1.25 | 2.65 (0.03) | 1.25 | 0.98 (0.44) | 1.00 |
| DMM CCR2-AggKO | 0.50 | 0.75 | 1.00 | 0.86 | |||||
| Subchondral bone thickness | DMM CCR2+/+ | 2.47 (0.02) | 3740 | 0.05 (0.96) | 3544 | 1.84 (0.09) | 6356 | 2.47 (0.02) | 6547 |
| DMM CCR2-AggKO | 3127 | 3421 | 4789 | 4705 | |||||
| Osteophyte Cartilage Quantification | DMM CCR2+/+ | 0.00 (1.00) | 48.00 | −3.33 (0.004) | 19.5 | −2.89 (0.008) | 15.50 | −0.05 (1.00) | 16.00 |
| DMM CCR2-AggKO | 46.05 | 38.90 | 41.00 | 17.00 | |||||
| Pain | Incapacitance Meter | Von-Frey Filaments* | |||||||
| Time PO/Stage | DMM CCR2+/+ vs. DMM CCR2-AggKO | DMM CCR2+/+ vs. DMM CCR2-AggKO | DMM CCR2+/+ vs. Contralateral CCR2+/+ | DMM CCR2-AggKO vs. Contralateral -CCR2-AggKO | |||||
| 4 wks/mild | 0.10 (0.00, 0.19) | 0.21 (−0.22,0.64) | 0.62 (0.13,1.11) | 0.2 (−0.21,0.61) | |||||
| 8 wks/moderate | 0.10 (−0.02, 0.23) | 0.11 (−0.32,0.55) | 0.38 (−0.11,0.87) | 0.37 (−0.09,0.83) | |||||
| 12 wks/severe | 0.13 (0.01, 0.26) | −0.25 (−0.61,0.11) | 0.55 (0.19,0.91) | 0.28 (−0.1,0.66) | |||||
| 16 wks/severe | 0.15 (0.05, 0.26) | 0.26 (−0.37,0.89) | 0.68 (0.3,1.07) | 0.67 (0.28,1.06) | |||||
| 20 wks/severe | 0.23 (0.14, 0.31) | −0.42 (−0.81,−0.04) | 1.00 (0.58,1.41) | 0.67 (0.19,1.15) | |||||
OA parameters: Data presented show median for each group at separate time points. Indicated z- and p-values were determined by Wilcoxon rank sum tests between DMM CCR+/+ and dMm CCR-AggKO groups separately at each time point, following adjustment for multiple comparisons (Benjamini-Hochberg). For more complete information, see Supplementary Table X.
Pain: Results are least-squares mean differences (and corresponding 95% CIs) from separate linear mixed effects models.