Table 2.
Effect of late CCR2 inactivation (4wks post-DMM) on OA assessment (N=8) in mouse CCR2-AggKO and CCR2+/+ knee joints.
| OA PARAMETERS | Week 8 | Week 12 | |||||||
|---|---|---|---|---|---|---|---|---|---|
| n | Z(pvalue) | median | mean (SD) | n | Z (pvalue) | Median | mean (SD) | ||
| ACS | CCR2+/+ | 8 | 2.16 (0.10) | 4.88 | 3.97 (1.95) | 8 | 1.52 (0.13) | 6.50 | 6.25 (0.95) |
| CCR2-AggKO | 8 | 0.94 | 2.16 (2.02) | 10 | 5.88 | 5.50 (1.29) | |||
| Saf-o | CCR2+/+ | 9 | 1.60 (0.05) | 3.75 | 3.56 (1.36) | 8 | 0.22 (0.82) | 5.50 | 4.97 (2.00) |
| CCR2-AggKO | 9 | 1.25 | 1.99 (1.69) | 10 | 5.86 | 5.24 (1.45) | |||
| Cartilage Quantification | CCR2+/+ | 8 | 0.05 (0.96) | 14849 | 14907 (2538) | 8 | −0.04 (0.96) | 4860 | 7655 (6398) |
| CCR2-AggKO | 8 | 13854 | 16038 (7093) | 10 | 8269 | 8221 (4809) | |||
| Osteophyte size | CCR2+/+ | 9 | −0.90 (0.41) | 0.63 | 0.49 (0.28) | 8 | −0.82 (0.41) | 1.13 | 1.10 (0.51) |
| CCR2-AggKO | 9 | 0.63 | 0.72 (0.35) | 10 | 1.25 | 1.27 (0.23) | |||
| Osteophyte maturity | CCR2+/+ | 9 | 0.00 (1.00) | 0.63 | 0.54 (0.28) | 8 | 2.48 (0.02) | 1.35 | 1.30 (0.27) |
| CCR2-AggKO | 9 | 0.50 | 0.57 (0.24) | 10 | 1.00 | 0.95 (0.26) | |||
| Subchondral bone thickness | CCR2+/+ | 8 | 1.97 (0.10) | 4850 | 4869 (509) | 8 | 0.00 (1.00) | 7387 | 7224 (1713) |
| CCR2-AggKO | 9 | 4125 | 4191 (598) | 10 | 7189 | 7298 (1417) | |||
| Osteophyte Cartilage quantification | CCR2+/+ | 6 | −0.58 (0.56) | 16.25 | 15.77 (5.57) | 6 | −2.89 (0.008) | 12.10 | 12.42 (6.79) |
| CCR2- AggKO | 8 | 18.8 | 20.58 (10.49) | 9 | 28.7 | 35.44 (15.03) | |||
Data presented show median, mean and standard deviation (SD), and number of animals in each group at 8 and 12 weeks. Indicated z- and p-values were determined by Wilcoxon rank sum tests between DMM CCR+/+ and DMM CCR-AggKO groups separately at each time point, following adjustment for multiple comparisons (Benjamini-Hochberg).