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. 2022 Nov 17;13:7038. doi: 10.1038/s41467-022-34808-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a PAS staining of wild-type mouse livers in different ages (4w, 4 weeks; 6w, 6 weeks; 8w, 8 weeks). The percentage of positive area is measured by Image J and shown on the right. Bar, 50 μm. n = 5 animals. Data are presented as mean values +/− SEM. *p < 0.05; ***p < 0.001 by one-way ANOVA. b Transmission electron microscope pictures of wild-type mouse livers in different ages. Bar: top, 4 μm; bottom, 2 μm. This experiment was repeated independently with similar results at least 3 times. c Hepatic glycogen content at different ages, as micrograms per milligram of tissue, n = 5 animals. Data are presented as mean values +/− SEM. Two-sided Student’s t test was performed to determine a difference among groups. ***p = 0.0006. d The relationship between relative fold of m6A mRNA and hepatic glycogen content at different age samples. Black, 4w; blue, 8w. n = 10 animals. Linear regression was performed to determine a difference. Source data are provided as a Source data file.