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. 2022 Nov 17;13:7038. doi: 10.1038/s41467-022-34808-2

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© The Author(s) 2022

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 2 — a Relative expression levels (FPKM) of five key genes in m6A writing or erasing from a mouse liver-associated GEO dataset (GSE58827). n = 3 independent samples. Data are presented as mean values +/− SEM. Two-way ANOVA was performed to determine a difference among columns within a gene. *p < 0.05. b Western blotting assay of Mettl3 protein level in wild-type mouse livers from different ages. This experiment was repeated independently with similar results at least 3 times. c Hepatic glycogen content at different ages, as micrograms per milligram of tissue, n = 5 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between each column. ns, not significant; ***p < 0.001. d PAS staining of livers in 8-week-old mice with different Mettl3 genotypes. The percentage of positive area is measured by Image J and shown on the right. WT wild type, HET heterozygous, cKO conditional knockout (Albumin-cre). Bar, 50 μm. n = 5 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between each column. ns, not significant; ***p < 0.001. e Transmission electron microscope pictures of livers in 8-week-old mouse with indicated Mettl3 genotypes. Bar: top, 4 μm; bottom, 2 μm. This experiment was repeated independently with similar results at least 3 times. f Serum glucose level of 8-week-old mice with different Mettl3 genotypes. n = 5 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between each column. ns, not significant; **p < 0.01. g Exhaustive swimming assay of 8-week-old mice with different Mettl3 genotypes. n = 6 animals. Data are presented as mean values +/− SEM. One-way ANOVA was performed to determine a difference between each column. ns, not significant; ***p < 0.001. Source data are provided as a Source data file. See also Supplementary Figs. 1 and 2.