TABLE 1.

Comparison between the extent of ¹⁴C-palmitate oxidation and the number of CFU of M. tuberculosis (H37Rv) in a suspension exposed to MPO^a

Exptl condition	CFU (106 [%])	14CO2 from 14C-palmitate (cpm [%]) at:
		3 days	6 days
H37Rv (0.2 × 105)		372	543 ± 100
H37Rv (0.2 × 106)	0.180 (100)	480 (100)	802 ± 138 (100)
H37Rv + MPO	0.170 (94)	405 (84)	947 ± 210 (118)
H37Rv + H2O2	0.150 (83)	307 (64)	537 ± 188 (67)
H37Rv + MPO + H2O2	0.025 (13.8)	386 (80)	745 ± 228 (92.8)

^aValues (CFU, 10⁶) are the means of two separate experiments. The concentration of MPO was 10 GU/10⁶ M. tuberculosis cells, and the concentration of H₂O₂ was 0.5 mM. The preincubation period was 90 min. CFU were counted after 3 weeks. The amount of ¹⁴CO₂ released from ¹⁴C-palmitate was evaluated after 3 or 6 days, and the values are the means (± standard deviations) of either three experiments carried out with two center-well-equipped flasks per condition per experiment or of one experiment carried out with three separate center-well-equipped flasks per condition. The background value (already subtracted) in the absence of bacteria was 55 ± 15 cpm. See Materials and Methods for more details.