TABLE 2.
Sensitivity of two M. tuberculosis strains and one M. avium strain to peroxidase activitiesa
Exptl condition | % Growth of:
|
||
---|---|---|---|
M. tuberculosis H37Rv | M. tuberculosis H19b | M. avium 485 | |
Control | 100 | 100 A | 100 |
MPO + H2O2 | 15 | 29 ± 14 B | 45 |
MPO | 92 | 80 ± 34 C | 100 |
HRP + H2O2c | 89 | NDd | ND |
H2O2 | 100 | 80 ± 15 D | 52 |
Values are expressed as percentages (± standard deviations) of growth, taking the number of CFU of untreated bacteria as 100%. The concentration of MPO was 3 GU/106 M. tuberculosis and M. avium cells, and the concentration of HRP was 18 GU/106 M. tuberculosis cells. Two experiments each were conducted for M. tuberculosis H37Rv and M. avium 485; three experiments were conducted for M. tuberculosis H19. See Materials and Methods for more details.
B versus A, P < 0.015 (significant); C versus A and A versus D, not significant; B versus C, P < 0.002 (very significant). All results by two-tailed Student’s t test.
These experiments were carried out in 0.02 M sodium phosphate buffer (pH 7.0) containing 0.067 M sodium sulfate and 0.2 mM NaI.
ND, not done.