TABLE 3.
Effects of different halides on the microbicidal activity of MPO on M. tuberculosis H37Rva
| Halide added | % Growth of M. tuberculosis H37Rv in the presence of:
|
||
|---|---|---|---|
| MPO | H2O2 | MPO + H2O2 | |
| None | 100 | 95 | 101 |
| Cl− | 92 | 99 | 40 |
| I− | 90 | 92 | 122 |
| Br− | 100 | 101 | 100 |
a
Values (means of two separate experiments) are expressed as percentages of growth taking the number of CFU exposed to MPO alone, in the absence of halide, as 100%. The concentrations used were as follows: MPO, 5 GU/106 M. tuberculosis cells; H2O2, 0.5 mM; Cl−, 100 mM; I−, 0.2 mM; Br−, 0.2 mM. There was a 60-min period of incubation. See Materials and Methods for more details.