Fig. 8. Total microbial diversity across vertebrate hindguts and within multiple body sites of fish.

a Hindgut microbiota samples from 569 species of vertebrates were rarified to 5000 reads and unique or shared ASVs determined for each class. b The percentage of unique ASVs only found in a given class (not shared in other classes) as compared to the total ASVs within that class. c Rarefaction of cumulative gamma diversity as a function of unique vertebrate species. Included is a single fish species, S. japonicus, sampled over 3 years “black dots” and the unrarefied FMP samples which had detectable bacteria in all four body sites (gill, skin, midgut, and hindgut). d Gamma diversity of 68 fish species across four body sites. e Percentage of unique ASVs associated with a given body site across the 68 fish species. f Rarefaction curve of increasing gamma diversity (inclusive of four body sites) as a function of increasing fish species. ASV amplified sequence variant, 5k 5000.