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Western blot showing expression of OapA in whole-cell lysates. (A) H. influenzae strains. Lanes: 1, Eagan (type b); 2, H229 (Eagan oapA mutant); 3, Rd; 4, H209 (Rd oapA mutant); 5, H217 (H209 corrected oapA mutant); 6, H135 (nontypeable); 7, H142 (nontypeable); 8, H233 (nontypeable); and 9, H487 (H233 oapA mutant). (B) E. coli strains. Lane: 1, OapA-positive DH5α; 2, OapA-negative DH5α. Molecular size markers are indicated at the left and expressed in kilodaltons.
