FIG. 5.

Immunocytochemistry of the binding of rOapA to Chang epithelial cells. Confluent epithelial cell layers were incubated with 20 μg of biotinylated BSA (A) or rOapA (B) per ml for 1 h at 37°C. The bound proteins were identified with streptavidin peroxidase as described in the Materials and Methods section. Original magnification, ×200.