TABLE 2.
Binding of E. coli strains expressing or not expressing OapA to Chang epithelial cells
E. coli strains and phenotype | No. of bacteria bounda
|
|
---|---|---|
CFU/well | % of inoculum | |
DH5α(pUC4) | 1.0 × 106 ± 0.2 × 106 | 3.9 ± 0.76 |
DH5α(pE214) OapA+ | 4.65 × 106 ± 0.2 × 106 | 12.2 ± 0.53b |
DH5α(pE219) OapA− | 1.23 × 106 ± 0.5 × 106 | 4.1 ± 1.74 |
DH5α(pEL1) OapA+ | 5.35 × 106 ± 0.7 × 106 | 13.5 ± 1.76b |
Mean ± standard deviation of at least three separate experiments done in triplicate.
P < 0.01 by two-tailed t test compared to OapA− strains.