Skip to main content
. 2022 Nov 17;13(11):965. doi: 10.1038/s41419-022-05398-0

Fig. 2. miR-769-5p genetic silencing reverses the effects of MS-275 in the expression of TGFβRI and other MMT-related markers.

Fig. 2

A RT-PCR showing the effect of miR-769-5p, miR3607-3p and miR5195-3p specific silencing on TGFBRI mRNA expression in MCs from PD patients. miR-16 was used for normalization. Bars represent the mean ± SEM of duplicate determinations in three to five independent experiments. B RT-PCR showing the effect of miR-769-5p genetic silencing on the expression of TGFBRI, ACTA2, COL1A1, TGFβ1, SMAD2, SMAD3 and PAI-1 in the presence of MS-275. Quantitative RT-PCR was performed on total RNA. L34 mRNA levels were used for normalization. Bars represent the mean ± SEM of duplicate determinations in three to four independent experiments. C WB showing the expression of TGFBRI, PAI-I, SMAD2 andSMAD3 upon genetic silencing of miR-769-5p. HSP90 was used as a loading control. Data are representative of three independent experiments. D Dual luciferase reporter gene assay showing the specific targeting of TGFBRI, SMAD2, SMAD3 and PAI-1, by miR-769-5p Bars represent the mean ± SEM of duplicate determinations in four to five independent experiments performed in MeT5A cells.