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. 2022 Nov 17;13(11):965. doi: 10.1038/s41419-022-05398-0

Fig. 3. miR-769-5p ectopic expression is sufficient to inhibit TGFβ1-induced expression of MMT molecular markers.

Fig. 3

A RT-PCR experiments showing the expression of miR-769-5p and of TGFBRI, TGFβ1, PAI-1, Occludin-1, SMAD2, SMAD3, ACTA2, and Col1A1 mRNA upon ectopic expression of miR-769-5p. Quantitative RT-PCR was performed on total RNA. L34 mRNA levels were used for normalization. Bars represent the mean ± SEM of duplicate determinations in three to six independent experiments. B WB showing the expression of SMAD2, TGFBRI, SMAD3 and PAI-I upon ectopic expression of miR-769-5p. Tubulin was used as a loading control. Data are representative of three independent experiments. C Immunofluoresce of MCs from PD patients transfected with mimic miR-769-5p. Fixed cells were stained with antibodies against type I COLLAGEN, FN-1 and α-SMA. Data are representative of three independent experiments.