Effects of, glucose, palmitic acid, and cholesterol in various concentrations on the human HSCs survival. The MTT assay results show the viability of the cells treated to different cholesterol, glucose, and palmitic acid concentrations after 24 h. obtained results are shown with mean ± SEM. Analysis was done by Tukey test, one-way ANOVA, and GraphPad Prism 8 program. (**p < 0.01, ***p < 0.001, ****p < 0.0001)