FIG. 2.

Effects of various inhibitors on C. jejuni 81-176 internalization into INT407 (A) and Caco-2 (B) cells. Data showing the concentration dependence of inhibition are presented in panel C. One hour prior to the addition of bacteria to the monolayer, the epithelial cells were incubated with no inhibitor (ni), with 2 μM cytochalasin D (CD), 10 μM colchicine (Co), 50 μM vinblastine (VB), 50 μM vincristine (VC), or with the various inhibitors at the concentrations listed in panel C. Each inhibitor was maintained throughout the 2-h invasion period. Relative percent invasiveness was determined as recovery in the presence of inhibitors divided by recovery in the absence of inhibitors (i.e., 100% relative invasiveness). S. typhi Ty2W served as an MF-dependent invasive control; the asterisk denotes that Ty2W internalization was decreased by >99% in the presence of CD. Results are presented as the mean of at least three separate experiments ± SE, shown as bars above or below the mean.