FIG. 6.

Effect of inhibition of host cell dynein on C. jejuni 81-176 invasion ability. Thirty minutes before bacteria were added to the monolayer, the epithelial cells were incubated either with no inhibitor (ni) or with 1, 10, or 100 μM Na₃VO₄. This dynein inhibitor was maintained throughout the 2-h invasion period. Relative percent invasiveness was determined as recovery in the presence of inhibitors divided by recovery in the absence of inhibitors (i.e., 100% relative invasiveness). C. jejuni invasion in the absence of inhibitor was significantly (P < 0.01) reduced by monolayer pretreatment with 100 μM Na₃VO₄. S. typhi Ty2W served as an invasive control which is not affected by dynein inhibition.