FIG. 7.

Representative immunofluorescence microscopic images of C. jejuni- and S. typhi-infected INT407 cells to examine bacterial association with dynein. Infected host cells were prepared and immunolabeled as described in Materials and Methods, using differential fluorescence labels. In these photomicrographs, all pixels of light derived from Texas red-X (dynein; A and C) and FITC (bacteria; B and D) are shown in white. (A) Dynein observed in host cells infected with C. jejuni for 1 h. (B) Corresponding microscopic field showing immunolabeled C. jejuni. The positions of several bacteria in panel B are indicated in panel A by arrows and demonstrate C. jejuni colocalization with dynein. (C) Host cell dynein observed after 1 h of infection with S. typhi. (D) Corresponding microscopic field showing immunolabeled S. typhi. Invading S. typhi did not colocate with dynein. Bar markers represent 1 μm.