Table 4.
Coded coefficient and significance level (p-value) of the model and independent variables on haloacid dehalogenase production by E. coli BL21 (DE3)/pET-hakp1.
| Terms and Variables | Coded Coefficient | p-value |
|---|---|---|
| Model | - | 0.003 |
| Linear | - | 0.265 |
| Induction temperature(°C) | 0.0147 | 0.458 |
| Duration of induction (hours) | 0.0148 | 0.456 |
| IPTG concentration (mM) | -0.0351 | 0.095 |
| Square | - | 0.000 |
| Induction temperature (°C)∗Induction temperature (°C) | -0.1116 | 0.000 |
| Duration of induction (hours)∗Duration of induction (hours) | -0.0432 | 0.042 |
| IPTG concentration (mM)∗IPTG concentration (mM) | -0.0853 | 0.001 |
| Interaction | - | 0.950 |
| Induction temperature (°C)∗Duration of induction (hours) | -0.0024 | 0.925 |
| Induction temperature (°C)∗IPTG concentration (mM) | 0.0037 | 0.884 |
| Duration of induction (hours)∗IPTG concentration (mM) | 0.0138 | 0.591 |