Fig. 3. scRNA-seq of d20 WT and CHD7^KO/KO organoids revealed critical role of CHD7 in otic development.

a, b Uniform manifold approximation and projection (UMAP) plot and cluster annotation of FACS-isolated PAX2^nG-positive cells from d20 WT and CHD7^KO/KO organoids as grouped by cluster (a) or by genotype (b). Dotted line highlights the two otic progenitor clusters. Data represent 22,390 cells. c Split-violin plots of key genes dysregulated in CHD7^KO/KO otic progenitors compared to the WT otic progenitors.