Fig. 8. The WT-CHD7^KO/KO chimeric organoid approach is designed to test whether signaling ligands from WT cells can rescue the CHD7^KO/KO phenotypes in neighboring mutant tissues.

a Heatmap showing the scaled log-normalized expression of several cells signaling ligand genes in d20 WT and CHD7^KO/KO otic progenitors. b WT-CHD7^KO/KO chimeric organoid culture strategy for supplying CHD7^KO/KO mutant cells with diffusible morphogens and cell surface signaling ligands from neighboring and nearby AAVS1^mT-labeled WT cells.