TABLE 1.
Determination of subcellular fraction purity by mitochondrial (SR) and plasma membrane (APD) marker enzyme activity
| Day | Fraction | % of total enzyme activity (mean ± SD [n = 3])
|
|||
|---|---|---|---|---|---|
| Liver
|
Spleen
|
||||
| SR | APD | SR | APD | ||
| 0 | Nuclear | 2.4 ± 1.9 | 2.8 ± 2.5 | 11.4 ± 1.0 | 4.2 ± 0.1 |
| Mitochondrial | 92.5 ± 8.6 | 18.7 ± 15.6 | 66.9 ± 0.9 | 35.4 ± 3.8 | |
| Cytosolic | 0.1 ± 0.06 | 6.5 ± 5.2 | 11.4 ± 1.8 | 6.8 ± 5.7 | |
| Plasma membrane | 4.9 ± 6.6 | 76.2 ± 16.1 | 10.3 ± 1.7 | 53.6 ± 1.9 | |
| 1 | Nuclear | 4.5 ± 3.1 | 2.8 ± 1.3 | 7.0 ± 2.1 | 24.0 ± 1.4 |
| Mitochondrial | 77.8 ± 9.5 | 34.2 ± 8.8 | 74.0 ± 3.3 | 31.6 ± 2.8 | |
| Cytosolic | 1.1 ± 0.9 | 4.4 ± 0.9 | 6.4 ± 1.3 | 1.1 ± 0.5 | |
| Plasma membrane | 16.6 ± 11.4 | 58.6 ± 11.1 | 12.6 ± 0.1 | 43.3 ± 3.8 | |
| 3 | Nuclear | 4.2 ± 6.3 | 2.1 ± 0.1 | 4.8 ± 2.3 | 9.1 ± 7.0 |
| Mitochondrial | 78.4 ± 7.5 | 38.5 ± 1.7 | 79.8 ± 7.9 | 41.0 ± 4.8 | |
| Cytosolic | 3.9 ± 6.8 | 6.8 ± 0.8 | 5.3 ± 4.1 | 0.9 ± 0.6 | |
| Plasma membrane | 13.2 ± 6.5 | 52.7 ± 1.1 | 10.1 ± 1.4 | 49.0 ± 1.6 | |
| 6 | Nuclear | 1.9 ± 3.6 | 1.7 ± 0.3 | 9.8 ± 0.2 | 13.2 ± 0.4 |
| Mitochondrial | 93.1 ± 8.8 | 52.9 ± 7.4 | 66.2 ± 3.3 | 35.5 ± 11.6 | |
| Cytosolic | 2.0 ± 3.1 | 5.2 ± 0.2 | 13.7 ± 7.9 | 0.8 ± 0.1 | |
| Plasma membrane | 3.3 ± 2.1 | 40.5 ± 7.3 | 10.3 ± 4.4 | 50.5 ± 11.1 | |
| 9 | Nuclear | 1.2 ± 2.3 | 1.9 ± 0.9 | 3.6 ± 1.8 | 13.0 ± 3.5 |
| Mitochondrial | 89.2 ± 4.4 | 43.4 ± 8.0 | 76.1 ± 7.3 | 37.6 ± 1.2 | |
| Cytosolic | 1.7 ± 3.0 | 4.8 ± 3.1 | 9.7 ± 2.1 | 1.4 ± 0.6 | |
| Plasma membrane | 7.9 ± 1.1 | 50.7 ± 4.2 | 10.6 ± 3.3 | 48.0 ± 5.3 | |
| 26 | Nuclear | 1.6 ± 2.7 | 1.0 ± 0.1 | 2.3 ± 0.4 | 12.0 ± 0.4 |
| Mitochondrial | 81.9 ± 9.9 | 36.3 ± 2.8 | 83.3 ± 5.3 | 15.5 ± 0.1 | |
| Cytosolic | 2.5 ± 4.2 | 4.8 ± 3.8 | 2.4 ± 0.3 | 1.6 ± 0.1 | |
| Plasma membrane | 13.7 ± 2.5 | 57.9 ± 2.6 | 12.0 ± 4.4 | 70.9 ± 0.6 | |