TABLE 2.
Subcellular distribution of hsp60 in spleen and liver fractions of Listeria-infected mice, determined by densitometry readings
| Fraction | % of total hsp60 expression (mean ± SD [n = 3])
|
|||||
|---|---|---|---|---|---|---|
| Day 0 | Day 1 | Day 3 | Day 6 | Day 9 | Day 26 | |
| Liver | ||||||
| Nuclear | 2.1 ± 4.0 | 22.0 ± 4.7 | 11.5 ± 5.9 | 13.1 ± 7.5 | 18.4 ± 7.5 | 14.0 ± 1.4 |
| Mitochondrial | 47.2 ± 13.9 | 37.0 ± 19.0 | 37.2 ± 12.4 | 34.1 ± 6.9 | 40.3 ± 21.4 | 10.4 ± 0.1 |
| Cytoplasmic | 24.1 ± 9.6 | 26.7 ± 19.8 | 39.4 ± 3.8 | 21.4 ± 5.3 | 16.9 ± 5.0 | 13.1 ± 6.3 |
| PMa | 26.7 ± 9.8 | 14.3 ± 5.5 | 11.8 ± 4.4 | 31.5 ± 3.4 | 24.4 ± 18.4 | 62.5 ± 7.9 |
| PM as % of mitochondria | 55.6 | 38.6 | 31.7 | 92.4 | 60.5 | 600.9 |
| Spleen | ||||||
| Nuclear | 6.2 ± 3.4 | 2.0 ± 2.0 | 15.2 ± 14.3 | 18.3 ± 11.9 | 9.2 ± 8.4 | 0.1 ± 0.5 |
| Mitochondrial | 82.5 ± 0.6 | 94.3 ± 21.3 | 67.2 ± 15.9 | 46.4 ± 5.1 | 66.7 ± 12.2 | 65.1 ± 0.2 |
| Cytoplasmic | 0.7 ± 2.8 | 2.1 ± 4.0 | 2.1 ± 2.7 | 15.4 ± 2.8 | 2.6 ± 0.1 | 0.1 ± 4.9 |
| PM | 10.5 ± 0.1 | 1.5 ± 2.9 | 15.5 ± 1.3 | 19.9 ± 8.0 | 21.5 ± 10.9 | 34.7 ± 4.1 |
| PM as % of mitochondria | 12.7 | 1.6 | 23.1 | 42.9 | 32.2 | 53.1 |
a
PM, plasma membrane.