Table 1.
Kinetic data for ScMqnA and its mutants
| Enzyme (mutant) | Km (μM) | vmax (nkat/mg) | kcat (s-1) |
|---|---|---|---|
| ScMqnA (wildtype) | 121 ± 38 | 7.63 ± 0.65 | 0.24 ± 0.02 |
| ScMqnA (Asn17Asp) | − | − | − |
| ScMqnA (Asn17Ser) | − | − | − |
| ScMqnA (Asn17Ala) | 257 ± 87 | 7.88 ± 0.81 | 0.25 ± 0.02 |
| ScMqnA (Ser86Ala) | − | − | − |
| ScMqnA (Tyr242Phe) | 377 ± 106 | 17.65 ± 2.15 | 0.56 ± 0.06 |
Every datapoint was measured three times (n = 3), and standard errors were calculated by the fitting software. The kinetics of Asn17Asp, Asn17Ser, and Ser86Ala could not be determined due to their very weak activity in the assay.