Immunofluorescence micrographs of 3LL (left column) and EA.hy926 cell lines (right column) after 24-h incubation with 10 and 25 μM Fisetin NCs (a, e), and free Fisetin at the same respective concentration (b, f). Control groups: (c, g) P407, and (d, h) DMSO at the same ratio as the Fisetin NCs and the free Fisetin, respectively. FITC (green) and DAPI (blue) indicate the tubulins and nuclei, respectively. (i) The corresponding form factor for each system, calculated as percentage of the control group, i.e. the endothelial cells grown in the culture medium only. The data were processed with GraphPad Prism version 9 from immunofluorescence micrographs. Data are mean of triplicate. (For interpretation of the references to colour in this figure legend, the reader is referred to the web version of this article.)